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Effects of olive pomace oil (OPO), high oleic sunflower oil (HOSO) and sunflower oil (SO) on endothelial <t> function biomarkers. </t>
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Effects of olive pomace oil (OPO), high oleic sunflower oil (HOSO) and sunflower oil (SO) on endothelial <t> function biomarkers. </t>
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Effects of olive pomace oil (OPO), high oleic sunflower oil (HOSO) and sunflower oil (SO) on endothelial <t> function biomarkers. </t>
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Effects of olive pomace oil (OPO), high oleic sunflower oil (HOSO) and sunflower oil (SO) on endothelial function biomarkers.
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Effects of olive pomace oil (OPO), high oleic sunflower oil (HOSO) and sunflower oil (SO) on endothelial function biomarkers.
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Effects of olive pomace oil (OPO), high oleic sunflower oil (HOSO) and sunflower oil (SO) on endothelial function biomarkers.
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SwitchGear Genomics human tgf-β3, nppa, negative control (scrambled), or positive control (actin) luciferase reporter construct
Transcriptome analyses reveal Smyd1 to be a transcriptional repressor of a core set of developmental genes. Transcriptome analyses were performed separately on right ventricles (RV) and left ventricles (LV) from mice with deletion of Smyd1 at 2 wk and 9 wk after removal from tamoxifen diet (controls were normal diet-fed littermates). A: all genes measured in both groups, RV and LV, are displayed in heat map format (red is upregulation, green downregulation, and black statistically unchanged) and clustered according to similar behavior. 2 clusters were defined as indicated, and the functionality of genes in those clusters was determined by gene ontology (GO) analysis. The networks for cluster 1 (B) and 2 (C) for the biological process ontology are shown. In the network figures, node size indicates the P value, and linkage between two terms indicates the relatedness, as determined by κ statistics. The color indicates functional groups with each group represented by their most significant leading term. All terms shown in the network image of cluster 1 are filtered at P < 0.005; those in cluster 2 are filtered at P < 0.0001. Bioinformatic analyses of transcripts with altered expression in the LV, at week 2 (D) and week 9 (E) after Tmx treatment, show significant enrichment in genes involved in extracellular matrix remodeling, fibrosis, and transcriptional repression. MF, molecular function; BP = biological process; CC, cellular component; KEGG, KEGG analysis; ITP, Interpro analysis. Microarray results for several of these transcripts were subsequently validated by RT-PCR (F: all those we attempted to validate are shown; n = 3–6/group; *P < 0.05 vs no tamoxifen group). Chromatin immunoprecipitation (ChIP) and qPCR for Smyd1 (using FLAG antibody) show enrichment in the promoter region of target genes tgfbeta3 (G) and <t>nppa</t> (H); however, no corresponding enrichment of histone H3 lysine K4 trimethylation was detected in these regions (bottom). *P ≤ 0.05. <t>I:</t> <t>luciferase</t> reporter assay using the tgfbeta3 and nppa promoters confirms that Smyd1 acts as a transcriptional repressor by inhibiting transcription of these genes; n = 6/group; *P < 0.05. J: as a negative control, Smyd1 is enriched by ChIP-PCR at neither β-tubulin nor β-actin using primers shown previously to target the regulatory regions upstream of these genes [−3 kb for β-tubulin (24), −73 bp for β-actin (29)].
Human Tgf β3, Nppa, Negative Control (Scrambled), Or Positive Control (Actin) Luciferase Reporter Construct, supplied by SwitchGear Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Yanke Inc desktop high-speed centrifuge tg-16
Transcriptome analyses reveal Smyd1 to be a transcriptional repressor of a core set of developmental genes. Transcriptome analyses were performed separately on right ventricles (RV) and left ventricles (LV) from mice with deletion of Smyd1 at 2 wk and 9 wk after removal from tamoxifen diet (controls were normal diet-fed littermates). A: all genes measured in both groups, RV and LV, are displayed in heat map format (red is upregulation, green downregulation, and black statistically unchanged) and clustered according to similar behavior. 2 clusters were defined as indicated, and the functionality of genes in those clusters was determined by gene ontology (GO) analysis. The networks for cluster 1 (B) and 2 (C) for the biological process ontology are shown. In the network figures, node size indicates the P value, and linkage between two terms indicates the relatedness, as determined by κ statistics. The color indicates functional groups with each group represented by their most significant leading term. All terms shown in the network image of cluster 1 are filtered at P < 0.005; those in cluster 2 are filtered at P < 0.0001. Bioinformatic analyses of transcripts with altered expression in the LV, at week 2 (D) and week 9 (E) after Tmx treatment, show significant enrichment in genes involved in extracellular matrix remodeling, fibrosis, and transcriptional repression. MF, molecular function; BP = biological process; CC, cellular component; KEGG, KEGG analysis; ITP, Interpro analysis. Microarray results for several of these transcripts were subsequently validated by RT-PCR (F: all those we attempted to validate are shown; n = 3–6/group; *P < 0.05 vs no tamoxifen group). Chromatin immunoprecipitation (ChIP) and qPCR for Smyd1 (using FLAG antibody) show enrichment in the promoter region of target genes tgfbeta3 (G) and <t>nppa</t> (H); however, no corresponding enrichment of histone H3 lysine K4 trimethylation was detected in these regions (bottom). *P ≤ 0.05. <t>I:</t> <t>luciferase</t> reporter assay using the tgfbeta3 and nppa promoters confirms that Smyd1 acts as a transcriptional repressor by inhibiting transcription of these genes; n = 6/group; *P < 0.05. J: as a negative control, Smyd1 is enriched by ChIP-PCR at neither β-tubulin nor β-actin using primers shown previously to target the regulatory regions upstream of these genes [−3 kb for β-tubulin (24), −73 bp for β-actin (29)].
Desktop High Speed Centrifuge Tg 16, supplied by Yanke Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Transcriptome analyses reveal Smyd1 to be a transcriptional repressor of a core set of developmental genes. Transcriptome analyses were performed separately on right ventricles (RV) and left ventricles (LV) from mice with deletion of Smyd1 at 2 wk and 9 wk after removal from tamoxifen diet (controls were normal diet-fed littermates). A: all genes measured in both groups, RV and LV, are displayed in heat map format (red is upregulation, green downregulation, and black statistically unchanged) and clustered according to similar behavior. 2 clusters were defined as indicated, and the functionality of genes in those clusters was determined by gene ontology (GO) analysis. The networks for cluster 1 (B) and 2 (C) for the biological process ontology are shown. In the network figures, node size indicates the P value, and linkage between two terms indicates the relatedness, as determined by κ statistics. The color indicates functional groups with each group represented by their most significant leading term. All terms shown in the network image of cluster 1 are filtered at P < 0.005; those in cluster 2 are filtered at P < 0.0001. Bioinformatic analyses of transcripts with altered expression in the LV, at week 2 (D) and week 9 (E) after Tmx treatment, show significant enrichment in genes involved in extracellular matrix remodeling, fibrosis, and transcriptional repression. MF, molecular function; BP = biological process; CC, cellular component; KEGG, KEGG analysis; ITP, Interpro analysis. Microarray results for several of these transcripts were subsequently validated by RT-PCR (F: all those we attempted to validate are shown; n = 3–6/group; *P < 0.05 vs no tamoxifen group). Chromatin immunoprecipitation (ChIP) and qPCR for Smyd1 (using FLAG antibody) show enrichment in the promoter region of target genes tgfbeta3 (G) and <t>nppa</t> (H); however, no corresponding enrichment of histone H3 lysine K4 trimethylation was detected in these regions (bottom). *P ≤ 0.05. <t>I:</t> <t>luciferase</t> reporter assay using the tgfbeta3 and nppa promoters confirms that Smyd1 acts as a transcriptional repressor by inhibiting transcription of these genes; n = 6/group; *P < 0.05. J: as a negative control, Smyd1 is enriched by ChIP-PCR at neither β-tubulin nor β-actin using primers shown previously to target the regulatory regions upstream of these genes [−3 kb for β-tubulin (24), −73 bp for β-actin (29)].
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Image Search Results


Effects of olive pomace oil (OPO), high oleic sunflower oil (HOSO) and sunflower oil (SO) on endothelial  function biomarkers.

Journal: Foods

Article Title: Olive Pomace Oil versus High Oleic Sunflower Oil and Sunflower Oil: A Comparative Study in Healthy and Cardiovascular Risk Humans

doi: 10.3390/foods11152186

Figure Lengend Snippet: Effects of olive pomace oil (OPO), high oleic sunflower oil (HOSO) and sunflower oil (SO) on endothelial function biomarkers.

Article Snippet: Regarding endothelial function biomarkers, endothelial nitric oxide synthase (eNOS, SEA868Hu), E-selectin (SEA029Hu) and P-selectin (SEA569Hu) plasma concentrations were determined in duplicate by ELISA (Cloud-Clone Kit Corp., Katy, TX, USA) using a Bio-Tek ® Synergy™ HT Multi-Detection Microplate Reader controlled by BioTek ® Gen5 version 2.01.14 software (BioTek Instruments, Winooski, VT, USA).

Techniques:

Effects of olive pomace oil (OPO), high oleic sunflower oil (HOSO) and sunflower oil (SO) on oxidation and antioxidant  biomarkers.

Journal: Foods

Article Title: Olive Pomace Oil versus High Oleic Sunflower Oil and Sunflower Oil: A Comparative Study in Healthy and Cardiovascular Risk Humans

doi: 10.3390/foods11152186

Figure Lengend Snippet: Effects of olive pomace oil (OPO), high oleic sunflower oil (HOSO) and sunflower oil (SO) on oxidation and antioxidant biomarkers.

Article Snippet: Regarding endothelial function biomarkers, endothelial nitric oxide synthase (eNOS, SEA868Hu), E-selectin (SEA029Hu) and P-selectin (SEA569Hu) plasma concentrations were determined in duplicate by ELISA (Cloud-Clone Kit Corp., Katy, TX, USA) using a Bio-Tek ® Synergy™ HT Multi-Detection Microplate Reader controlled by BioTek ® Gen5 version 2.01.14 software (BioTek Instruments, Winooski, VT, USA).

Techniques:

Effects of olive pomace oil (OPO), high oleic sunflower oil (HOSO) and sunflower oil (SO) on endothelial function biomarkers.

Journal: Foods

Article Title: Olive Pomace Oil versus High Oleic Sunflower Oil and Sunflower Oil: A Comparative Study in Healthy and Cardiovascular Risk Humans

doi: 10.3390/foods11152186

Figure Lengend Snippet: Effects of olive pomace oil (OPO), high oleic sunflower oil (HOSO) and sunflower oil (SO) on endothelial function biomarkers.

Article Snippet: Regarding endothelial function biomarkers, endothelial nitric oxide synthase (eNOS, SEA868Hu), E-selectin (SEA029Hu) and P-selectin (SEA569Hu) plasma concentrations were determined in duplicate by ELISA (Cloud-Clone Kit Corp., Katy, TX, USA) using a Bio-Tek ® Synergy™ HT Multi-Detection Microplate Reader controlled by BioTek ® Gen5 version 2.01.14 software (BioTek Instruments, Winooski, VT, USA).

Techniques:

Transcriptome analyses reveal Smyd1 to be a transcriptional repressor of a core set of developmental genes. Transcriptome analyses were performed separately on right ventricles (RV) and left ventricles (LV) from mice with deletion of Smyd1 at 2 wk and 9 wk after removal from tamoxifen diet (controls were normal diet-fed littermates). A: all genes measured in both groups, RV and LV, are displayed in heat map format (red is upregulation, green downregulation, and black statistically unchanged) and clustered according to similar behavior. 2 clusters were defined as indicated, and the functionality of genes in those clusters was determined by gene ontology (GO) analysis. The networks for cluster 1 (B) and 2 (C) for the biological process ontology are shown. In the network figures, node size indicates the P value, and linkage between two terms indicates the relatedness, as determined by κ statistics. The color indicates functional groups with each group represented by their most significant leading term. All terms shown in the network image of cluster 1 are filtered at P < 0.005; those in cluster 2 are filtered at P < 0.0001. Bioinformatic analyses of transcripts with altered expression in the LV, at week 2 (D) and week 9 (E) after Tmx treatment, show significant enrichment in genes involved in extracellular matrix remodeling, fibrosis, and transcriptional repression. MF, molecular function; BP = biological process; CC, cellular component; KEGG, KEGG analysis; ITP, Interpro analysis. Microarray results for several of these transcripts were subsequently validated by RT-PCR (F: all those we attempted to validate are shown; n = 3–6/group; *P < 0.05 vs no tamoxifen group). Chromatin immunoprecipitation (ChIP) and qPCR for Smyd1 (using FLAG antibody) show enrichment in the promoter region of target genes tgfbeta3 (G) and nppa (H); however, no corresponding enrichment of histone H3 lysine K4 trimethylation was detected in these regions (bottom). *P ≤ 0.05. I: luciferase reporter assay using the tgfbeta3 and nppa promoters confirms that Smyd1 acts as a transcriptional repressor by inhibiting transcription of these genes; n = 6/group; *P < 0.05. J: as a negative control, Smyd1 is enriched by ChIP-PCR at neither β-tubulin nor β-actin using primers shown previously to target the regulatory regions upstream of these genes [−3 kb for β-tubulin (24), −73 bp for β-actin (29)].

Journal: American Journal of Physiology - Heart and Circulatory Physiology

Article Title: The chromatin-binding protein Smyd1 restricts adult mammalian heart growth

doi: 10.1152/ajpheart.00235.2016

Figure Lengend Snippet: Transcriptome analyses reveal Smyd1 to be a transcriptional repressor of a core set of developmental genes. Transcriptome analyses were performed separately on right ventricles (RV) and left ventricles (LV) from mice with deletion of Smyd1 at 2 wk and 9 wk after removal from tamoxifen diet (controls were normal diet-fed littermates). A: all genes measured in both groups, RV and LV, are displayed in heat map format (red is upregulation, green downregulation, and black statistically unchanged) and clustered according to similar behavior. 2 clusters were defined as indicated, and the functionality of genes in those clusters was determined by gene ontology (GO) analysis. The networks for cluster 1 (B) and 2 (C) for the biological process ontology are shown. In the network figures, node size indicates the P value, and linkage between two terms indicates the relatedness, as determined by κ statistics. The color indicates functional groups with each group represented by their most significant leading term. All terms shown in the network image of cluster 1 are filtered at P < 0.005; those in cluster 2 are filtered at P < 0.0001. Bioinformatic analyses of transcripts with altered expression in the LV, at week 2 (D) and week 9 (E) after Tmx treatment, show significant enrichment in genes involved in extracellular matrix remodeling, fibrosis, and transcriptional repression. MF, molecular function; BP = biological process; CC, cellular component; KEGG, KEGG analysis; ITP, Interpro analysis. Microarray results for several of these transcripts were subsequently validated by RT-PCR (F: all those we attempted to validate are shown; n = 3–6/group; *P < 0.05 vs no tamoxifen group). Chromatin immunoprecipitation (ChIP) and qPCR for Smyd1 (using FLAG antibody) show enrichment in the promoter region of target genes tgfbeta3 (G) and nppa (H); however, no corresponding enrichment of histone H3 lysine K4 trimethylation was detected in these regions (bottom). *P ≤ 0.05. I: luciferase reporter assay using the tgfbeta3 and nppa promoters confirms that Smyd1 acts as a transcriptional repressor by inhibiting transcription of these genes; n = 6/group; *P < 0.05. J: as a negative control, Smyd1 is enriched by ChIP-PCR at neither β-tubulin nor β-actin using primers shown previously to target the regulatory regions upstream of these genes [−3 kb for β-tubulin (24), −73 bp for β-actin (29)].

Article Snippet: After 24 h, the cells were transfected with 75 ng of human TGF-β3, Nppa, negative control (scrambled), or positive control (actin) luciferase reporter construct (SwitchGear Genomics) using FuGene HD (SwitchGear Genomics) at a 6:1 ratio to DNA and incubated for 48 h. Luciferase activity was assayed using the LightSwitch Luciferase assay reagent (SwitchGear Genomics) according to the manufacturer's instructions and measured using a BioTek Synergy Neo HTS Multi-Mode microplate reader.

Techniques: Functional Assay, Expressing, Microarray, Reverse Transcription Polymerase Chain Reaction, Chromatin Immunoprecipitation, Luciferase, Reporter Assay, Negative Control